Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Pedigrees of the Six Families with TNHP and Sanger Sequencing Confirming the Identified Mutations in TRPV6 that Show Complete Cosegregation with Phenotype

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques: Sequencing

Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Pre- and Postnatal Clinical Findings in Six Subjects with TRPV6 Variants

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques:

Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Functional Analysis of TRPV6 Variants

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques: Functional Assay

Journal: Cell Proliferation

Article Title: H3 relaxin protects against calcium oxalate crystal‐induced renal inflammatory pyroptosis

doi: 10.1111/cpr.12902

Figure Lengend Snippet: H3 relaxin reduces ATP and reactive oxygen species (ROS) synthesis through the RXFP1‐cAMP pathway. A, ROS analysis of the treated tubular epithelial cells (TECs) with or without the pretreatment of H3 relaxin and 3‐isobutyl‐1‐methylxanthine (IBMX). n = 3, data expressed as means ± SEM, ## P < .01 vs control group, * P < .05 vs CaOx group, ** P < .01 vs CaOx group. B, Mean fluorescence intensity of the ROS detected TECs, as a supplement to the overexpressed ROS percentage. n = 3, data expressed as means ± SEM, P < .05, comparison principles unchanged. C, cAMP levels of treated TECs were measured using enzyme‐linked immunosorbent assay. n = 3, data expressed as means ± SEM, ** P < .01 vs control group. D, Western blot analysis of PKA in the treated TECs, in the absence or presence of H3 relaxin. n = 3, representative image is shown. E, cAMP levels in the treated TECs in the absence or presence of H3 relaxin and IBMX. n = 3, data expressed as means ± SEM, ** P < .01 vs control group. F, Intracellular ATP and eATP levels in the treated TECs in the absence or presence of H3 relaxin and IBMX. n = 3, data expressed as means ± SEM, ## P < .01 vs control group, * P < .05 vs CaOx group, ** P < .01 vs CaOx group. G, Western blot analysis of RXFP‐1 and inflammatory pyroptosis‐related proteins in the treated TECs, in the absence or presence of H3 relaxin and si‐RXFP‐1 pretreatment. n = 3 per group, representative images are shown

Article Snippet: The following primary antibodies were used: ASC (sc‐514414), PKA (sc‐390548), P2X 7 R (sc‐514962) and RXFP‐1 (sc‐293228) from Santa Cruz Biotech; cleaved caspase‐1 (4199/89332) and pro‐caspase‐1 (3866) from Cell Signaling Technology; IL‐1β (WL00891) and NLRP3 (WL02635) from Wanleibio (Wanleibio); cleaved GSDMD ([ab215203; Abcam], [sc‐393656; Santa Cruz Biotech]); IL‐18 (10663‐1‐AP; ProteinTech); and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (AC002; ABclonal).

Techniques: Control, Fluorescence, Comparison, Enzyme-linked Immunosorbent Assay, Western Blot